BDGP Resources
Laboratory Methods
- Preparation of BAC
DNA Clones
A protocol is available from BACPAC Resources - DNA
Isolation Protocols for P1 Clones
These protocols give standard or modified-Qiagen methods to isolate P1 clone DNA. - In Situ
Hybridization Using Digoxigenin Labeled Probes
The BDGP Cytogenetics Core uses this protocol to localize P1s, BACs, and P element insertion sites on the polytene chromosome map.
- 96-well RNA In
Situ Hybridization Protocol
This is the protocol used to generate the embryonic whole-mount in situ expression patterns. It is an adaptation of standard protocols to a 96-well format, and includes PCR protocols for pBS and quantification of RNA. - Protocols
Used to Isolate Membrane-Bound Polysomes and to Construct the
Normalized CK cDNA Library
Isolation of mRNA from Drosophila rough endoplasmic reticulum
Normalization of a Drosophila cDNA library by hybridization to gDNA beads - PCR protocol for genomic DNA
- Sizing cDNA
PCR products
This protocol gives the PCR method used to size cDNA clone inserts. - pOT2 Vector
- pOTB7 Vector
- pOTB7D Vector
- pFLC-I Vector
- Expression Vectors
- In-Fusion
Protocol
Method for cloning full-length ORFs using the Clontech PCR cloning system - PCR Amplification of cDNAs fromBacterial Cultures: DGC/pOT2
- Large Inserts: PCR Amplification of DGC cDNAs greater than 3.5kb
- PCR
Amplification of D. melogaster UniGene
Collection
Using plasmid DNA as template