BDGP Resources
Expression Vectors
Four widely used Drosophila expression vectors have been
converted into vectors that will accept ORFs from the ExpressR-NS
and ExpressR-O clones to generate both 5' and 3' fusions. These
vectors are used for inducible protein expression in tissue culture
cells under the metallothionein-inducible promoter (A and B) and in
transgenic flies under GAL4-inducible UAS promoter (C and D). The
vectors each contain a 549 bp fragment encoding the Tandem Affinity
Purification (TAP) tag positioned to create N-terminal and
C-terminal fusion proteins. The TAP tag provides an efficient and
reliable way to recover protein complexes present at low cellular
expression levels under native conditions. A description of the
parent vectors that these originate from can be found here.
Download the fasta file for Cloning vector pUAST-NTAPBDBD here
